A DNA-binding protein GATA1 with a biological unit FOG1 Zinc finger Protein molecule is ‘synergistic’ to the region of the X chromosome which occurred at a exome splice site X-linked involving the GATA-type zinc finger domain.

The human ERYF1 gene (summary) NF-E1 DNA-binding protein GATA1, locus Xp11.23 [§§] containing 2 ‘finger’ motifs referred to as ERYF1 of an erythroid-specific gene. The cDNA for the human ERYF1 gene is almost identical to that of chicken and mouse GATA1 gene consisting of 2 zinc finger’ type motifs its activator domain contains the binding sites for protein GATA1 and the CACCC (HS2)^ region. FOG is specific to this complex corresponding cDNA and interacts with element in the beta-globin IVS2 promoter from hemoglobin protein subunit promoters (alpha-chain gene‡, gamma, epsilon^ and  (embryonic), a switch from fetal to adult haemoglobin -or- relative to the T to C substitution of fetal hemoglobin (HPFH), implications for fetal hemoglobin – HbF“) distinct for erythroid (INHBA) and megakaryocyte differentiation, in vertabrate though, the N- and C-terminal thirds of the human protein.  Friend of GATA-1, FOG1; ZFPM1, zinc finger protein region a coregulator of the GATA1 associations facilitates a chromatin  locus control region(LCR)modifying proximity fetal to adult (gamma) to beta globin including the erythroid (EKLF krüpple-like) factor DNAse1^ histone  hypersensativesite (HS)^ locus (LCR) GATA1 establishes, facilitates interactions with immunoprecipitation, cross-regulatory roles reduced histone, acetylation and antagonism (EKLF-FlI-1) mechanisms. PU.1 – of the Ets family is ‘synergistic‘ to the major basic protein, (MBP) handles bistability in the erythroid-‘myeloid switch « directed by PU.1,’ influenced DNA binding and is  involved with MZF-1 (myeloid zinc finger 1), it interacts with the ‘C-terminal zinc finger « (CF)’ of GATA1. A bipotential function in multiple contexts (erythroid versusmegakaryocytic myeloid cells, GATA1 switches myeloid cell fate into eosinophils)° as two multi-protein complexes when segregated into two types (factor P-TEFb) one of the characteristics of (TAL-1, T-cell acute-) leukemic (SCL) stem cells is both types in circulating blood, for both the downregulation of GATA-1 and with the upregulation of GATA-2 (3q21)° that CD34␠ has the transcription capacity observed in immature hematopoietic progenitor stem cells, specific regions of each (Sequencing of FOG1 with GATA1 and GATA2), requires intact DNA-binding domains.  The C-terminal zinc finger (CF) basic tail shares, in an antagonistic fashion ‘mutations‘ in exon 2‡ (-GATA1s is a shorter GATA1 isoform (sf) found in DS (Down syndrome) a transient leukemia (TL)-AMKL) that lacks the transactivation‘” domain, in cis-acting GATA element, identification requires intact long forms (lf) of NF-E1 DNA-binding domain. Two novel zinc-finger domains demonstrate that the NFE1 gene cDNA-binding protein is assigned the human locus located in Xp11.23, required for normal megakaryocytic and erythroid development. A mutation in the FOG1-GATA1 N-terminal zinc finger (N-finger of leukemic cell (Igs)-immunoglobulins) or lacking the N-terminal activation the binding of Fog1 and the N-finger in the DNA face of Fog1, with non X-linked associations (16q2224) if different clinical entities linking to X-linked (X is any amino acid, substitution in the DNA-binding (Nf) region) thrombocytopenia in males-(XLTT*’-GATA1) with anemia low platelet levels traces discernable steps as embryos with a defect in forming erythroid burst-forming units BFU-E ☞ (summary – of all DNA that is transcribed which occurred at a exome splice site), to Minimal residual disease MRD – (cancer, “preleukemia” – myeloproliferative disorder (TMD), myeloid leukaemia-AML, SCL° and megakaryocytic AMKL) the GATA1-HS2-modified vector allowed remission in blood component and heme (Protoporphyrinogen) at the seventh GATA site in exon 1*’/intron-7° as a cofactor involving 6 non-coding exons and transactivation by USF1 and GATA1. A DNA Cytosine mechanism ara-c (Arabinofuranosylcytosine) short (sf) and (lf) long forms is used to kill these megakaryocytic cancer cells; clarifies that GATA-1 controls genes that manipulate the cell cycle and apoptotic cell death underlying normal (PI3K) and pathologic (PU.1) erythropoiesis – ‘differentiation’ is (FKBP12) lacking basal expression‘” in contrast to Bcl when Bcl-X(L) is cleaved by caspases. Anti-apoptotic Hsp70 protects GATA-1 during the switchingª of the erythroleukemia␠ cells that fail to complete maturation, proteolysis undergoing cell death in both the megakaryocytic and erythroid cells, established that phospholipase C (PLC)ª is involved in the signalling pathway(PI3K)/Akt equally expressed ‘as’ a probable negative FOG regulator, interacts with the PU.1 related Ets domain of  glycoprotein (GP)(1) VI*’ by expressing thrombopoietin activation of platelets in megakaryocytic cell lines, expressing both Fli-1 and GATA-1. A weak loss of aspartate in the amino-N-terminal zinc finger (Nf) loop GATA1’s three base substitution mutations results in incomplete megakaryocyte/platelet maturation as assessed by the DNA demethylating agent 5-azacytidine, activity in the presence of ara-c which occurred at a exome splice site. GATA1 appears to interact with RNA-mediated basal expression against these pathways, associated protein or mammalian targetsclarified that the basal transcription apparatus with transcription factors“ appears to interact with an HS2 region mutated in its GATA motif GATA1s a shorter GATA1 isoform.  

sequence [AT]GATA[AG] upper left 4 Angstroms of PDB 1GAT in this 4 Angstrom PDB 3VD6 r
Figure 1: PDB 1y0j-a  (MMDB ID: 31470; Mus musculus A). superimposed on -3vd6 4 Angstrons ogf DNA, six finger Znf DNA potential (‘X is any amino acid, substitution’) to co-ordinate C2H2 znf-1y0j-B (Protein chain B, MMDB ID: 31470), and the original structure of DNA_GATA1_HUMAN PDB: 1Y0J_uniprot/P15976 ProteinModelPortal P15976. / PDB: _3vd6; Names: GATA1 :ERYF1, GF1 with the consensus seqence [AT]GATA[AG] upper left DNA fragment seen in SPNA1  DNA binding  an essential determinant of specific GATA 1 Fig.2 binding, wraps around into the minor groove seen as the lower RNA representing PDB 1GAT in this single PDB 3VD6 rendering with PDB: 1YOJ- element-A DNA-binding protein GATA1 RNA Mus musculus eg. the red tail is the assumed Adjacent GATA DNA binding of PDB: 3DFV (Structure|id=PIRSF003027) Figure 2: 4 Angstroms of PDB 1GAT in this 4 Angstrom PDB 3VD6 rendering of 1YOJ-A RNA, modifyed to complete Fig.1. both are manually defined selected to provide The two zinc fingers functionality that contains 2 GATA-type zinc fingers (See; Figure 3: FOG1_B Zinc finger Protein (MMDB ID: 31470) has an absence of the PDB: 1YOJ- element- A DNA-binding protein GATA1 RNA thereby The two (Znf) fingers are functionally distinct bridging two separate DNA fragments (Structure|ids=PIRSF003027).
gata1 biological unit ara-C (Arabinofuranosylcytosine) Cytarabine (CID_6253; SDF File (.sdf)) = ara-c (MMDB ID: 23600 PDB ID: 1P5Z) Swiss PDB-viewer SPDBV
Figure 3: This incorporates PDB 1YOJ_A_B the Sructural basis of GATA1_A erythroid trascription factor and FOG1_B Zinc finger Protein (MMDB ID: 31470; Mus musculus A- Drosophila melanogaster-B) interactions with Human components of  Complexed With a molecue biological unit ara-C (Arabinofuranosylcytosine) Cytarabine (CID_6253; SDF File (.sdf)) = ara-c (MMDB ID: 23600 PDB ID: 1P5Z) short (sf) and (lf) long forms  2 ‘finger’ motifs of GATA 1 (lf) and FOG (sf) with  (FKBP12) basal expression PDB 2FAP_component A represented as the ligand surface partially framing the FOG heterodimer prevents formation of DNA component PDB: 1GAT-cDNA when lacking basal expression. This apparatus appears to interact with an HS2 region mutated in its GATA motif.
Zinc fingers as protein recognition motifs: structural basis for the GATA-1/Friend of GATA interaction 

Rendered with Swiss PDB-viewer SPDBV
about a horizontal axis of the Structures Image in the plane of the page
http://www.rcsb.org/pdb/explore/explore.do?pdbId=1Y0J
Refernce:
Mol Cell Biol. 2005 Feb;25(4):1215-27.
GATA1 function, a paradigm for transcription factors in hematopoiesis.
PMID:
    15684376
Swiss-pdb viewer software (http://www.expasy.org/spdbv/)

One Comment

  1. Posted October 1, 2013 at 1:04 am | Permalink | Reply

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